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Conceived and designed the experiments:

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These data suggest that plasma DNA in these cancer patients is largely derived from tumor. The concentration of cpDNA with the optimal ability to detect a mutation is Molecular analysis of these genomic aberrations is usually conducted on archival tumor tissue due to ethical and safety challenges associated with repeated biopsies.

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No patients with wildtype tumor tissue genotypes had mutations in their respective cpDNA. Eur J Surg Oncol Parameter No.

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